Improved diagnostics for helminth NTDs

DOLF researchers are not only performing clinical trials, large community trials and other epidemiological and intervention studies on helminths in the field they run also state of the art molecular helminthology laboratories and have access to core facilities for genome sequencing, proteomics and molecular imaging. Within the DOLF project, the team identified problems of currently used diagnostic tools and performed various studies to help to develop, evaluate and improve diagnostic methods for helminth parasites. The studies include:  


Capillaria Ova and Diagnosis of Trichuris trichiura Infection in Humans by Kato-Katz Smear, Liberia.
K Fischer, A Gankpala, L Gankpala, FK Bolay, KC Curtis, GJ Weil, PU Fischer
Emerg Infect Dis 2018;24(8):1551-1554 

Identification and characterization of Loa loa antigens responsible for cross-reactivity with rapid diagnostic tests for lymphatic filariasis.
MI Hertz, H Nana-Djeunga, J Kamgno, A Jelil Njouendou, V Chawa Chunda, S Wanji, PJ Budge
PLoS Negl Trop Dis 2018;12(11) 

Differential human gut microbiome assemblages during soil-transmitted helminth infections in Indonesia and Liberia.
BA Rosa, T Supali, L. Gankpala, Y Djuardi, E Sartono, Y Zhou, K Fischer, J Martin, R Tyagi, FK Bolay, PU Fischer, M Yazdanbakhsh, M Mitreva
Microbiome 2018;6(33)

A multi-center field study of two point-of-care tests for circulating Wuchereria bancrofti antigenemia in Africa.
CB Chesnais, NP Awaca-Uvon, FK Bolay, M Boussinesq, PU Fischer, L Gankpala, A Meite, F Missamou, SD Pion, GJ Weil
PLoS Negl Trop Dis 2017;11(9):1-15

Use of a novel portable 3D scanner to measure limb volume and circumference in patients with filarial lymphedema.
C Yahathugoda, M Weiler, R Rao, L De Silva, J Dixon, M Weerasooriya, GJ Weil, P Budge
Am J Trop Med Hyg 2017;97:1836-1842 

Familial aggregation and heritability of Wuchereria bancrofti infection.
CB Chesnais, A Sabbagh, SD Pion, F Missamou, A Garcia, MJ Boussinesq
JID 2016;214:587-594 

A Comparison of two tests for filarial antigenemia in areas in Sri Lanka and Indonesia with low-level persistence of lymphatic filariasis following mass drug administration.
TC Yahathugoda, T Supali, RU Rao, Y Djuardi, D Stefani, F Pical, PU Fischer, MM Lloyd, PH Premaratne, MV Weerasooriya, GJ Weil
Parasites & Vectors 2015;8:369 

Conventional parasitology and DNA-based diagnostic methods for onchocerciasis elimination programmes.
MM Lloyd, R Gilbert, NT Taha, GJ Weil, A Meite, IMM Kouakou, PU Fischer
Acta Trop 2015;146:114-118 

Diagnostic tools for onchocerciasis elimination programs.
J Vlaminck, PU Fischer, GJ Weil
Trends in Parasitology 2015;31:571-82 

Filarial Antigenemia and Loa loa Night Blood Microfilaremia in an Area Without Bancroftian Filariasis in the Democratic Republic of Congo.
DK Bakajika, MM Nigo, JP Lotsima, GA Masikini, K Fischer, MM Lloyd, GJ Weil, PU Fischer
Am J Trop Med Hyg 2014;91(6):1142-1148 

Laboratory and field evaluation of a new rapid test for detecting Wuchereria bancrofti antigen in human blood.
GJ Weil, KC Curtis, L Fakoli, K Fischer, L Gankpala, PJ Lammie, AC Majewski, S Pelletreau, KY Won, FK Bolay, PU Fischer
Am J Trop Med Hyg 2013;89(1):11-15 

Semi-quantitative scoring of an immunochromatographic test for circulating filarial antigen.
CB Chesnais, F Missamou, SDS Pion, J Bopda, F Louya, AC Majewski, GJ Weil, M Boussinesq
Am J Trop Med Hyg 2013;89(5):916-918